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eclipse 80i fluorescence microscope  (Nikon)


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    Structured Review

    Nikon eclipse 80i fluorescence microscope
    Eclipse 80i Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+80i+fluorescence+microscope/Objectives/bio_rxiv__64898__2026__03__20__713277-360-7-6
    Average 99 stars, based on 59909 article reviews
    eclipse 80i fluorescence microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: KCTD10 is a sensor for co-directional transcription-replication conflicts.
    Article Snippet: .. Coverslips were mounted onto slides and images were acquired on a Nikon eclipse 80i fluorescence microscope with a 63× objective. ..

    Article Title: Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis.
    Article Snippet: For microscopic imaging, the cells were incubated with Hoechst dye (SigmaAldrich) (1:1,000 in PBS) for nuclear staining. .. After washing, the cells were mounted onto a glass slide and imaged under an Eclipse 80i fluorescence microscope (Nikon) using fluorescence and differential interference contrast (DIC) modes. ..

    Article Title: Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis.
    Article Snippet: .. Mouse monoclonal anti-CD63 antibody (Santa Cruz Biotechnology) or isotype IgG (Santa Cruz Biotechnology) (both at 1:50 in 1% BSA/PBS) was added and incubated at 37 °C for 1 h. After washing with PBS, AlexaFlour488-conjugated donkey anti-mouse IgG (Invitrogen-Molecular Probes) (1:500 in 1% BSA/PBS) was added and incubated at 37 °C in the dark for 1 h. After washing, the CD63-positive cells were analyzed using the BD Accuri C6 flow cytometer relative to 10,000 cells or imaged under the Nikon Eclipse 80i fluorescence microscope. .. The fluorescence intensity of CD63 was measured from 100 cells/sample using NIS-Elements D software version 4.11 (Nikon).

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Cells were washed three times with 1X PBS, s tained with DAPI (D9542, Sigma-Aldrich, 4 μg/ml diluted in 1X PBS) and mounted with ProLong Gold Antifade mountant (P36930, Thermo Scientific). .. Immunofluorescence images were acquired by CoolSnap MYO camera of Nikon Eclipse 80i fluorescence microscope using a Plan Apo VC 60× Oil DIC N2 objective lens. .. Images with 100X magnification were acquired using a 100X oil objective lens of Nikon Eclipse Ti2 microscope.

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Cells were washed with 1X PBS, stained with DAPI (D9542, Sigma-Aldrich, 4 μg/ml diluted in 1X PBS) and mounted using ProLong Gold Antifade mountant (P36930, Thermo Scientific). .. IdU immunofluorescence foci were examined by Nikon Eclipse 80i fluorescence microscope using a 60× Oil DIC N2 objective lens. .. Images were quantified using CellProfiler 4.2.5 software and scatter plots were generated in GraphPad Prism 10.

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Slides were washed three times in PBST and mounted with Prolong Gold antifade mountant (P36930, ThermoFisher Scientific). .. DNA fibers were imaged by Nikon Eclipse 80i fluorescence microscope using a Plan Apo VC 60× Oil DIC N2 objective lens. .. Fibers were quantified in ImageJ ( https://imagej.net/ij/ ) and fork restart efficiency was determined by fractions of CldU-IdU (red-green) labeled fibers as a percentage of total CldU (red and red-green) labeled fibers.

    Article Title: KCTD10 is a sensor for co-directional transcription–replication conflicts
    Article Snippet: .. Coverslips were mounted onto slides and images were acquired on a Nikon eclipse 80i fluorescence microscope with a 63× objective. ..

    Article Title: PFAS Alter Thyroid Histology and Cellular Signaling In Vitro and In Vivo
    Article Snippet: .. Immunofluorescence imaging was performed using a CoolSNAP ES camera mounted on a Nikon Eclipse 80i fluorescence microscope with a 40× objective. ..

    Microscopy:

    Article Title: KCTD10 is a sensor for co-directional transcription-replication conflicts.
    Article Snippet: .. Coverslips were mounted onto slides and images were acquired on a Nikon eclipse 80i fluorescence microscope with a 63× objective. ..

    Article Title: Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis.
    Article Snippet: For microscopic imaging, the cells were incubated with Hoechst dye (SigmaAldrich) (1:1,000 in PBS) for nuclear staining. .. After washing, the cells were mounted onto a glass slide and imaged under an Eclipse 80i fluorescence microscope (Nikon) using fluorescence and differential interference contrast (DIC) modes. ..

    Article Title: Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis.
    Article Snippet: .. Mouse monoclonal anti-CD63 antibody (Santa Cruz Biotechnology) or isotype IgG (Santa Cruz Biotechnology) (both at 1:50 in 1% BSA/PBS) was added and incubated at 37 °C for 1 h. After washing with PBS, AlexaFlour488-conjugated donkey anti-mouse IgG (Invitrogen-Molecular Probes) (1:500 in 1% BSA/PBS) was added and incubated at 37 °C in the dark for 1 h. After washing, the CD63-positive cells were analyzed using the BD Accuri C6 flow cytometer relative to 10,000 cells or imaged under the Nikon Eclipse 80i fluorescence microscope. .. The fluorescence intensity of CD63 was measured from 100 cells/sample using NIS-Elements D software version 4.11 (Nikon).

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Cells were washed three times with 1X PBS, s tained with DAPI (D9542, Sigma-Aldrich, 4 μg/ml diluted in 1X PBS) and mounted with ProLong Gold Antifade mountant (P36930, Thermo Scientific). .. Immunofluorescence images were acquired by CoolSnap MYO camera of Nikon Eclipse 80i fluorescence microscope using a Plan Apo VC 60× Oil DIC N2 objective lens. .. Images with 100X magnification were acquired using a 100X oil objective lens of Nikon Eclipse Ti2 microscope.

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Cells were washed with 1X PBS, stained with DAPI (D9542, Sigma-Aldrich, 4 μg/ml diluted in 1X PBS) and mounted using ProLong Gold Antifade mountant (P36930, Thermo Scientific). .. IdU immunofluorescence foci were examined by Nikon Eclipse 80i fluorescence microscope using a 60× Oil DIC N2 objective lens. .. Images were quantified using CellProfiler 4.2.5 software and scatter plots were generated in GraphPad Prism 10.

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Slides were washed three times in PBST and mounted with Prolong Gold antifade mountant (P36930, ThermoFisher Scientific). .. DNA fibers were imaged by Nikon Eclipse 80i fluorescence microscope using a Plan Apo VC 60× Oil DIC N2 objective lens. .. Fibers were quantified in ImageJ ( https://imagej.net/ij/ ) and fork restart efficiency was determined by fractions of CldU-IdU (red-green) labeled fibers as a percentage of total CldU (red and red-green) labeled fibers.

    Article Title: KCTD10 is a sensor for co-directional transcription–replication conflicts
    Article Snippet: .. Coverslips were mounted onto slides and images were acquired on a Nikon eclipse 80i fluorescence microscope with a 63× objective. ..

    Article Title: PFAS Alter Thyroid Histology and Cellular Signaling In Vitro and In Vivo
    Article Snippet: .. Immunofluorescence imaging was performed using a CoolSNAP ES camera mounted on a Nikon Eclipse 80i fluorescence microscope with a 40× objective. ..

    Incubation:

    Article Title: Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis.
    Article Snippet: .. Mouse monoclonal anti-CD63 antibody (Santa Cruz Biotechnology) or isotype IgG (Santa Cruz Biotechnology) (both at 1:50 in 1% BSA/PBS) was added and incubated at 37 °C for 1 h. After washing with PBS, AlexaFlour488-conjugated donkey anti-mouse IgG (Invitrogen-Molecular Probes) (1:500 in 1% BSA/PBS) was added and incubated at 37 °C in the dark for 1 h. After washing, the CD63-positive cells were analyzed using the BD Accuri C6 flow cytometer relative to 10,000 cells or imaged under the Nikon Eclipse 80i fluorescence microscope. .. The fluorescence intensity of CD63 was measured from 100 cells/sample using NIS-Elements D software version 4.11 (Nikon).

    Flow Cytometry:

    Article Title: Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis.
    Article Snippet: .. Mouse monoclonal anti-CD63 antibody (Santa Cruz Biotechnology) or isotype IgG (Santa Cruz Biotechnology) (both at 1:50 in 1% BSA/PBS) was added and incubated at 37 °C for 1 h. After washing with PBS, AlexaFlour488-conjugated donkey anti-mouse IgG (Invitrogen-Molecular Probes) (1:500 in 1% BSA/PBS) was added and incubated at 37 °C in the dark for 1 h. After washing, the CD63-positive cells were analyzed using the BD Accuri C6 flow cytometer relative to 10,000 cells or imaged under the Nikon Eclipse 80i fluorescence microscope. .. The fluorescence intensity of CD63 was measured from 100 cells/sample using NIS-Elements D software version 4.11 (Nikon).

    Immunofluorescence:

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Cells were washed three times with 1X PBS, s tained with DAPI (D9542, Sigma-Aldrich, 4 μg/ml diluted in 1X PBS) and mounted with ProLong Gold Antifade mountant (P36930, Thermo Scientific). .. Immunofluorescence images were acquired by CoolSnap MYO camera of Nikon Eclipse 80i fluorescence microscope using a Plan Apo VC 60× Oil DIC N2 objective lens. .. Images with 100X magnification were acquired using a 100X oil objective lens of Nikon Eclipse Ti2 microscope.

    Article Title: The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells
    Article Snippet: Cells were washed with 1X PBS, stained with DAPI (D9542, Sigma-Aldrich, 4 μg/ml diluted in 1X PBS) and mounted using ProLong Gold Antifade mountant (P36930, Thermo Scientific). .. IdU immunofluorescence foci were examined by Nikon Eclipse 80i fluorescence microscope using a 60× Oil DIC N2 objective lens. .. Images were quantified using CellProfiler 4.2.5 software and scatter plots were generated in GraphPad Prism 10.

    Article Title: PFAS Alter Thyroid Histology and Cellular Signaling In Vitro and In Vivo
    Article Snippet: .. Immunofluorescence imaging was performed using a CoolSNAP ES camera mounted on a Nikon Eclipse 80i fluorescence microscope with a 40× objective. ..

    Imaging:

    Article Title: PFAS Alter Thyroid Histology and Cellular Signaling In Vitro and In Vivo
    Article Snippet: .. Immunofluorescence imaging was performed using a CoolSNAP ES camera mounted on a Nikon Eclipse 80i fluorescence microscope with a 40× objective. ..



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